製品の概要

  • 製品名Anti-IMPDH1 antibody
    IMPDH1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IMPDH1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (RSKKGKLPIV NDCDELVAII ARTDLKKNRD YPLASKDSQK QLLCGAAVGT) of Human IMPDH1 (NP_899066).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • 組織特異性IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • パスウェイPurine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • 関連疾患Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
  • 配列類似性Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMDH1_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 1 antibody
    • IMP dehydrogenase 1 antibody
    • IMPD 1 antibody
    • IMPD antibody
    • IMPD1 antibody
    • IMPDH 1 antibody
    • IMPDH I antibody
    • IMPDH-I antibody
    • Impdh1 antibody
    • Inosine 5' monophosphate dehydrogenase 1 antibody
    • Inosine monophosphate dehydrogenase 1 antibody
    • Inosine-5''-monophosphate dehydrogenase 1 antibody
    • LCA11 antibody
    • RP10 antibody
    • sWSS2608 antibody
    see all

Anti-IMPDH1 antibody 画像

  • Anti-IMPDH1 antibody (ab84957) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 27 kDa,31 kDa. We are unsure as to the identity of these extra bands.
  • ab84957 staining IMPDH1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84957 staining IMPDH1 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-IMPDH1 antibody (ab84957) 使用論文

ab84957 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human cancer xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH 9
Permeabilization No
Specification human cancer xenograft
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
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投稿 Jun 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"