Anti-IMPDH1 抗体 (ab33039)
Key features and details
- Rabbit polyclonal to IMPDH1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IMPDH1 antibody -
製品の詳細
Rabbit polyclonal to IMPDH1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Human IMPDH1 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab33038) -
特記事項
The immunogen used for this product shares 86% homology with IMPDH2. Cross-reactivity with this protein has not been confirmed experimentally.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33039の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
特記事項 |
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WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
組織特異性
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
パスウェイ
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
関連疾患
Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. -
配列類似性
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. - Information by UniProt
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参照データベース
- Entrez Gene: 3614 Human
- Entrez Gene: 23917 Mouse
- Entrez Gene: 362329 Rat
- Omim: 146690 Human
- SwissProt: P20839 Human
- SwissProt: P50096 Mouse
- SwissProt: D3ZLZ7 Rat
- Unigene: 654401 Human
see all -
別名
- IMDH1_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 1 antibody
- IMP dehydrogenase 1 antibody
see all
画像
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All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa -
All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 :NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab33039 は 5 報の論文で使用されています。
- Zhao JZ et al. MYBL2 regulates de novo purine synthesis by transcriptionally activating IMPDH1 in hepatocellular carcinoma cells. BMC Cancer 22:1290 (2022). PubMed: 36494680
- Xu X et al. Rewiring of purine metabolism in response to acidosis stress in glioma stem cells. Cell Death Dis 12:277 (2021). PubMed: 33723244
- Feng X et al. UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism. EMBO J 39:e102541 (2020). PubMed: 31975428
- Dang W et al. Inhibition of Calcineurin or IMP Dehydrogenase Exerts Moderate to Potent Antiviral Activity against Norovirus Replication. Antimicrob Agents Chemother 61:N/A (2017). PubMed: 28807916
- Kim HR et al. A p53-inducible microRNA-34a downregulates Ras signaling by targeting IMPDH. Biochem Biophys Res Commun 418:682-8 (2012). WB ; Human . PubMed: 22301190