製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IMPACT
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 270-320 (GILLGPDRFK HINNCARNIL VEKNYTNSPE ESSKALGKNK KVRKDKKRNE H) of Human IMPACT (NP_060909.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab95175 was affinity purified using an epitope specific to IMPACT immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能
    Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
  • 組織特異性
    Widely expressed. Expressed at high level in brain.
  • 配列類似性
    Belongs to the IMPACT family.
    Contains 1 RWD domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMPACT antibody
    • Impact homolog (mouse) antibody
    • Impact homolog antibody
    • impact RWD domain protein antibody
    • IMPCT_HUMAN antibody
    • Imprinted and ancient gene protein homolog antibody
    • MGC33718 antibody
    • Protein IMPACT antibody
    • RWD domain containing 5 antibody
    • RWDD5 antibody
    see all

画像

  • All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 36 kDa


    Exposure time : 30 seconds
  • Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献

ab95175 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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