Rabbit polyclonal to IMPACT
Synthetic peptide, corresponding to a region within amino acids 270-320 (GILLGPDRFK HINNCARNIL VEKNYTNSPE ESSKALGKNK KVRKDKKRNE H) of Human IMPACT (NP_060909.1).
HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab95175 was affinity purified using an epitope specific to IMPACT immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
Use at 2-5 µg/mg of lysate.
Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
Widely expressed. Expressed at high level in brain.
Belongs to the IMPACT family.
Contains 1 RWD domain.
Information by UniProt
Impact homolog (mouse) antibody
Impact homolog antibody
Western blot - IMPACT antibody (ab95175)
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 36 kDa Exposure time: 30 seconds
Immunoprecipitation - IMPACT antibody (ab95175)
All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml Lane 1 : IP with IMPACT antibody Lane 2 : IP with ab95175 Lane 3 : IP with control IgG
has not yet been referenced specifically in any publications.
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