製品の概要

  • 製品名Anti-IMPA2 antibody
    IMPA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IMPA2
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human IMPA2.

    (Peptide available as ab97986.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver tissue lysate and HepG2 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能Can use myo-inositol monophosphates, scylloinositol 1,4-diphosphate, glucose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Has been implicated as the pharmacological target for lithium Li(+) action in brain.
  • パスウェイPolyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • 配列類似性Belongs to the inositol monophosphatase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMP 2 antibody
    • IMP A2 antibody
    • IMP.18P antibody
    • IMP2 antibody
    • IMPA 2 antibody
    • Impa2 antibody
    • IMPA2_HUMAN antibody
    • IMPase 2 antibody
    • Inosine monophosphatase 2 antibody
    • Inositol 1 antibody
    • Inositol 1(or 4) monophosphatase 2 antibody
    • Inositol monophosphatase 2 antibody
    • Inositol monophosphatase 2 variant 1 antibody
    • Inositol monophosphatase 2 variant 2 antibody
    • Inositol(myo) 1(or 4) monophosphatase 2 antibody
    • Inositol-1(or 4)-monophosphatase 2 antibody
    • Myo inositol monophosphatase A2 antibody
    • Myo-inositol monophosphatase A2 antibody
    see all

Anti-IMPA2 antibody 画像

  • All lanes : Anti-IMPA2 antibody (ab73076) at 1/250 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 170 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab73076 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73706, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.
  • IHC image of IMPA2 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-IMPA2 antibody (ab73076) 使用論文

ab73076 has not yet been referenced specifically in any publications.

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