製品の概要

  • 製品名Anti-IMPA1 antibody
    IMPA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IMPA1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Non Human Primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human IMPA1.

    (Peptide available as ab74969.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue, Testis (Human) Tissue, SK N SH Whole Cell, Jurkat Whole Cell, Thymus (Rat) Tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates.
  • パスウェイPolyol metabolism; myo-inositol biosynthesis; myo-inositol from D-glucose 6-phosphate: step 2/2.
  • 配列類似性Belongs to the inositol monophosphatase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMP 1 antibody
    • IMP antibody
    • IMPA 1 antibody
    • IMPA antibody
    • IMPA1 antibody
    • IMPA1_HUMAN antibody
    • IMPase 1 antibody
    • IMPase antibody
    • Inositol 1(or 4) monophosphatase antibody
    • Inositol monophosphatase 1 antibody
    • Inositol monophosphatase antibody
    • Inositol(myo) 1(or 4) monophosphatase 1 antibody
    • Inositol-1(or 4)-monophosphatase 1 antibody
    • Lithium sensitive myo inositol monophosphatase A1 antibody
    • Lithium-sensitive myo-inositol monophosphatase A1 antibody
    see all

Anti-IMPA1 antibody 画像

  • All lanes : Anti-IMPA1 antibody (ab73074) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 49 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab73074 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-IMPA1 antibody (ab73074) 使用論文

ab73074 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"