製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 95 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 DZF domain.
  • 翻訳後修飾
    Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
    Methylated by protein arginine N-methyltransferase 1.
    Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
  • 細胞内局在
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CBTF antibody
    • Double stranded RNA binding protein 76 antibody
    • Double-stranded RNA-binding protein 76 antibody
    • DRBF antibody
    • DRBP76 antibody
    • ILF3 antibody
    • ILF3_HUMAN antibody
    • Interleukin enhancer binding factor 3 antibody
    • Interleukin enhancer-binding factor 3 antibody
    • M phase phosphoprotein 4 antibody
    • M-phase phosphoprotein 4 antibody
    • MPHOSPH4 antibody
    • MPP4 antibody
    • NF AT 90 antibody
    • NF-AT-90 antibody
    • NF110 antibody
    • NF90 antibody
    • NFAR antibody
    • Nuclear factor associated with dsRNA antibody
    • Nuclear factor of activated T cells 90 kDa antibody
    • Nuclear factor of activated T-cells 90 kDa antibody
    • TCP80 antibody
    • Translational control protein 80 antibody
    see all

画像

  • Anti-ILF3 antibody (ab89100) at 1 µg/ml + Human pancreas lysate at 50 µg

    Predicted band size : 95 kDa
    Observed band size : 80,95 kDa (why is the actual band size different from the predicted?)
  • Anti-ILF3 antibody (ab89100) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 95 kDa
    Observed band size : 95 kDa
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-ILF3 antibody (ab89100) at 1 µg/ml

    Lane 1 : 293T transfected cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 95 kDa
    Observed band size : 95 kDa
  • ab89100, at 3µg/ml, staining human ILF3 in small intestine, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence of ab89100, used at 10µg/ml, to ILF3 on HeLa cells.

参考文献

ab89100 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
85 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Primary macrophage)
Specification
Primary macrophage
Blocking step
Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. David Gregory

Verified customer

投稿 Mar 23 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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