製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能
    Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • 配列類似性
    Contains 1 DZF domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HGNC:6037 antibody
    • ILF 2 antibody
    • ilf2 antibody
    • ILF2_HUMAN antibody
    • Interleukin enhancer binding factor 2 antibody
    • Interleukin enhancer binding factor 2, 45kDa antibody
    • Interleukin enhancer-binding factor 2 antibody
    • MGC8391 antibody
    • NF 45 antibody
    • NF45 antibody
    • Nuclear factor of activated T cells 45 kDa antibody
    • Nuclear factor of activated T-cells 45 kDa antibody
    • PRO3063 antibody
    see all

画像

  • All lanes : Anti-ILF2 antibody (ab93838) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 100 kDa,22 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

プロトコール

参考文献

ab93838 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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