Anti-ILF2/NF45 抗体 (ab113205)
Key features and details
- Rabbit polyclonal to ILF2/NF45
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ILF2/NF45 antibody
ILF2/NF45 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ILF2/NF45 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human ILF2/NF45 aa 340-390.
Database link: NP_004506.2 -
ポジティブ・コントロール
- WB: HeLa, 293T and mouse NIH3T3 cell lysates. IHC: mouse renal cell carcinoma, human lung cancer
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113205の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 43 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Predicted molecular weight: 43 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA. -
配列類似性
Contains 1 DZF domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 425709 Chicken
- Entrez Gene: 539760 Cow
- Entrez Gene: 3608 Human
- Entrez Gene: 67781 Mouse
- Entrez Gene: 310612 Rat
- Entrez Gene: 394593 Xenopus tropicalis
- Omim: 603181 Human
- SwissProt: Q12905 Human
see all -
別名
- HGNC:6037 antibody
- ILF 2 antibody
- ilf2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labelling ILF1 / NF45 with ab113205 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling ILF1 / NF45 with ab113205 at 1/5000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-ILF2/NF45 antibody (ab113205) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 43 kDa
Exposure time: 10 seconds -
Detection of ILF2/NF45 by Western Blot of Immunprecipitate.
Lane 1: ab113205 at 1µg/ml staining ILF2/NF45 in HeLa whole cell lysate immunoprecipitated using ab113205 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab113205 は 2 報の論文で使用されています。
- Kazuma Y et al. ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells. Sci Rep 12:2278 (2022). PubMed: 35145187
- Marchesini M et al. ILF2 Is a Regulator of RNA Splicing and DNA Damage Response in 1q21-Amplified Multiple Myeloma. Cancer Cell 32:88-100.e6 (2017). PubMed: 28669490