製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Used in peptide ELISA against the immunogen. Detection limit >1/32,000
ChIP Use at an assay dependent concentration. PubMed: 22083952This antibody is suitable for ChIP at some binding sites, but may not be sensitive enough to detect all FOXK2 binding sites.
ICC/IF Use at an assay dependent dilution. PubMed: 20810654
WB Use a concentration of 2 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa).Can be blocked with Human ILF1 peptide (ab23094).

ターゲット情報

  • 機能
    Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • 組織特異性
    Expressed in both lymphoid and non-lymphoid cells.
  • 配列類似性
    Contains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • ドメイン
    The C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Cellular transcription factor ILF 1 antibody
      • Cellular transcription factor ILF-1 antibody
      • Cellular transcription factor ILF1 antibody
      • Forkhead box K2 antibody
      • Forkhead box protein K2 antibody
      • FOX K1 antibody
      • FOX K2 antibody
      • FOXK 1 antibody
      • FOXK 2 antibody
      • FOXK1 antibody
      • foxk2 antibody
      • FOXK2_HUMAN antibody
      • ILF 1 antibody
      • ILF antibody
      • Interleukin enhancer binding factor 1 antibody
      • Interleukin enhancer-binding factor 1 antibody
      see all

    画像

    • Western blot - Anti-ILF1 antibody - ChIP Grade (ab5298)
      Western blot - Anti-ILF1 antibody - ChIP Grade (ab5298)
      Anti-ILF1 antibody - ChIP Grade (ab5298) at 0.3 µg/ml + Human thymus lysate in RIPA buffer at 35 µg

      Predicted band size: 73 kDa



      ab5298 at a 2µg/ml concentration staining approximately 73 kDa ILF 1 in human heart lysate (30µg total protein per lane) by Western blot (ECL). Primary incubation was 1 hour.
    • Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody - ChIP Grade (ab5298)
      Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody - ChIP Grade (ab5298)Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.
      Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.

    参考文献

    This product has been referenced in:
    • Liu Y  et al. FOXK2 transcription factor suppresses ERa-positive breast cancer cell growth through down-regulating the stability of ERa via mechanism involving BRCA1/BARD1. Sci Rep 5:8796 (2015). Read more (PubMed: 25740706) »
    • Ji Z  et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res 42:6232-42 (2014). IP . Read more (PubMed: 24748658) »

    See all 4 Publications for this product

    ab5298 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    We just noticed that you quoted your antibody: Anti-ILF1 antibody - ChIP Grade (ab5298) as “ChIP-seq grade”. However, the antibody is pretty poor in ChIP and not suitable for ChIP-seq. We used a flag-tagged construct in the paper you quoted...

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    Thank you for your comment.

    This particular antibody has not been tested for ChIP in-house in our Lab. The application of ChIP and ChIP seq has been added from a specific reference PMID22083952.

    Ji Z et al. The forkhead transcriptio...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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