製品の概要
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製品名Anti-ILF1 antibody - ChIP Grade
ILF1 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to ILF1 - ChIP Grade
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アプリケーション適用あり: ELISA, ChIP, ICC/IF, WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide: TPPAAVREKGVQN, corresponding to C terminal amino acids 648-660 of Human ILF1.
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ポジティブ･コントロール
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab5298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Used in peptide ELISA against the immunogen. Detection limit >1/32,000 | |
| ChIP | Use at an assay dependent concentration. PubMed: 22083952This antibody is suitable for ChIP at some binding sites, but may not be sensitive enough to detect all FOXK2 binding sites. | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 20810654 | |
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa).Can be blocked with Human ILF1 peptide (ab23094). |
ターゲット情報
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機能Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
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組織特異性Expressed in both lymphoid and non-lymphoid cells.
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配列類似性Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
ドメインThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
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細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 3607 Human
- Omim: 147685 Human
- SwissProt: Q01167 Human
- Unigene: 591140 Human
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別名
- Cellular transcription factor ILF 1 antibody
- Cellular transcription factor ILF-1 antibody
- Cellular transcription factor ILF1 antibody
see all
Anti-ILF1 antibody - ChIP Grade 画像
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Western blot - ILF1 antibody (ab5298)Anti-ILF1 antibody - ChIP Grade (ab5298) at 0.3 µg/ml + Human thymus lysate in RIPA buffer at 35 µg
Predicted band size : 73 kDa
Primary incubation was 1 hour. -
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody - ChIP Grade (ab5298)Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
Anti-ILF1 antibody - ChIP Grade (ab5298) 使用論文
This product has been referenced in:
- Ji Z et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res 42:6232-42 (2014). IP . Read more (PubMed: 24748658) »
- Ji Z et al. The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation. Mol Cell Biol 32:385-98 (2012). WB, ChIP ; Human . Read more (PubMed: 22083952) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"