製品の概要

  • 製品名Anti-ILF1 antibody
    ILF1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to ILF1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to internal sequence amino acids 158-257 of Human ILF1. Target sequence used to make the antibody:SSEKREKQEA SESPVKAVQP HISPLTINIP DTMAHLISPL PSPTGTISAA NSCPSSPRGA GSSGYKVGRV MPSDLNLMAD NSQPENEKEA SGGDSPKDDS.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 69 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.
  • 組織特異性Expressed in both lymphoid and non-lymphoid cells.
  • 配列類似性Contains 1 FHA domain.
    Contains 1 fork-head DNA-binding domain.
  • ドメインThe C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cellular transcription factor ILF 1 antibody
    • Cellular transcription factor ILF-1 antibody
    • Cellular transcription factor ILF1 antibody
    • Forkhead box K2 antibody
    • Forkhead box protein K2 antibody
    • FOX K1 antibody
    • FOX K2 antibody
    • FOXK 1 antibody
    • FOXK 2 antibody
    • FOXK1 antibody
    • foxk2 antibody
    • FOXK2_HUMAN antibody
    • ILF 1 antibody
    • ILF antibody
    • Interleukin enhancer binding factor 1 antibody
    • Interleukin enhancer-binding factor 1 antibody
    see all

Anti-ILF1 antibody 画像

  • All lanes : Anti-ILF1 antibody (ab52795) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution

    Predicted band size : 69 kDa

Anti-ILF1 antibody (ab52795) 使用論文

ab52795 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab52795.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"