The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at a concentration of 0.5 µg/ml. antibody. In conjunction with
compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine IL9.
Neut: Use at a concentration of 0.006 - 0.012 µg/ml to yield one-half maximal inhibition [ND50] of the biological activity of murine IL9 (0.30 ng/ml).
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine IL9 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 16 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Supports IL-2 independent and IL-4 independent growth of helper T-cells.