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RabMAb

Anti-IL8 抗体 [EPR1116(2)] (ab110727)

製品の概要

  • 製品名
    Anti-IL8 antibody [EPR1116(2)]
    IL8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1116(2)] to IL8
  • アプリケーション
    適用あり: WBmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IL8 aa 1 to the C-terminus. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: IL8 recombinant protein.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab110727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能
      IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • 配列類似性
      Belongs to the intercrine alpha (chemokine CxC) family.
    • 翻訳後修飾
      Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • (Ala-IL-8)77 antibody
      • (Ser-IL-8)72 antibody
      • 9E3 antibody
      • Beta thromboglobulin like protein antibody
      • C-X-C motif chemokine 8 antibody
      • CEF-4 antibody
      • chemokine, CXC motif, ligand 8 antibody
      • CXCL8 antibody
      • Emoctakin antibody
      • GCP-1 antibody
      • GCP/IL-8 protein I antibody
      • GCP/IL-8 protein II antibody
      • GCP/IL-8 protein III antibody
      • GCP/IL-8 protein IV antibody
      • GCP/IL-8 protein V antibody
      • GCP/IL-8 protein VI antibody
      • GCP1 antibody
      • Granulocyte chemotactic protein 1 antibody
      • IL-8 antibody
      • IL-8(1-77) antibody
      • IL-8(9-77) antibody
      • IL8 antibody
      • IL8/NAP1 form I antibody
      • IL8/NAP1 form II antibody
      • IL8/NAP1 form III antibody
      • IL8/NAP1 form IV antibody
      • IL8/NAP1 form V antibody
      • IL8/NAP1 form VI antibody
      • IL8_HUMAN antibody
      • Inteleukin 8 antibody
      • LECT antibody
      • LUCT antibody
      • Lymphocyte-derived neutrophil-activating factor antibody
      • LYNAP antibody
      • MDNCF antibody
      • MDNCF-b antibody
      • MDNCF-c antibody
      • MONAP antibody
      • Monocyte-derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil-activating peptide antibody
      • NAF antibody
      • NAP-1 antibody
      • NAP1 antibody
      • Neutrophil activating peptide 1 antibody
      • Neutrophil activating protein 1 antibody
      • Neutrophil-activating factor antibody
      • Neutrophil-activating protein 1 antibody
      • Protein 3 10C antibody
      • Protein 3-10C antibody
      • SCYB8 antibody
      • Small inducible cytokine subfamily B member 8 antibody
      • T cell chemotactic factor antibody
      • T-cell chemotactic factor antibody
      • TSG1 antibody
      see all

    画像

    • Anti-IL8 antibody [EPR1116(2)] (ab110727) at 1/10000 dilution (purified) + Human interleukin 8 recombinant protein at 0.005 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-IL8 antibody [EPR1116(2)] (ab110727) at 1/1000 dilution (unpurified) + IL8 recombinant protein at 10 µg

      Predicted band size : 11 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Huang WM  et al. Antioxidant and anti-inflammatory effects of Astragalus polysaccharide on EA.hy926 cells. Exp Ther Med 6:199-203 (2013). WB ; Human . Read more (PubMed: 23935746) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Human Tissue sections (Tonsil)
    Specification
    Tonsil
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Jun 12 2013

    Thank you for your email. This inquiry makes more sense now. Currently we do not have anti IL8 products which had been tested in sELISA in combination with other antibodies. I am sorry I am unable to recommend any capture or detection antibody at th...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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