製品の概要

  • 製品名Anti-IL8 antibody
    IL8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL8
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
    交差が予測される動物種: Chimpanzee, Baboon, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human IL8.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in IL-8 Recombinant Protein within Western Blot as well as in humam brain astrocytoma formalin fixed paraffin embedded tissue section within Immunohistochemistry.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody 画像

  • Anti-IL8 antibody (ab84995) at 1 µg/ml + Recombinant Protein:IL-8 at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 11 kDa


    Exposure time : 30 seconds
  • IHC image of ab84995 staining in human brain astrocytoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84995, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IL8 antibody (ab84995) 使用論文

ab84995 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jun 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"