製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 17208399
WB 1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.
IHC-Fr Use at an assay dependent concentration. PubMed: 25120588
IP 1/10.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 配列類似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻訳後修飾Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody 画像

  • All lanes : Anti-IL8 antibody (ab7747) at 1 µg/ml

    Lane 1 : Recombinant human IL8 protein (ab73858) at 0.1 µg
    Lane 2 : Recombinant human IL8 protein (ab73858) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 5000 µg/ml
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-IL8 antibody (ab7747) 使用論文

This product has been referenced in:
  • Ulbrich F  et al. Argon mediates protection by interleukin-8 suppression via a TLR2/TLR4/STAT3/NF-?B pathway in a model of apoptosis in neuroblastoma cells in vitro and following ischemia-reperfusion injury in rat retina in vivo. J Neurochem 138:859-73 (2016). Read more (PubMed: 27167824) »
  • Yang N  et al. TNF-a receptor antagonist attenuates isoflurane-induced cognitive impairment in aged rats. Exp Ther Med 12:463-468 (2016). WB ; Rat . Read more (PubMed: 27347079) »

See all 15 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (Macaque Retina)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization No
Specification Macaque Retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Muller Cell Line MIO-M1)
Permeabilization Yes - 0.1% TritonX-100
Specification Muller Cell Line MIO-M1
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (HNSCC Tissue)
Antigen retrieval step Heat mediated
Permeabilization No
Specification HNSCC Tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Biswanath Majumder

Verified customer

投稿 Jul 01 2015

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde ab7747 bisher noch nicht in Durchflusszytometrie getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein sp...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply.

In theory it is possible to perform IHC-P with a directly conjugated antibody. This will also mean that the amplification that is achieve by adding the secondary is lost.

So my advise would be to only do it...

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Thank you for your inquiry.

I am happy to confirm that the recommended protocol in IHC is the following:

Antigen retrieval: 5 min proteolytic enzyme (competitor), 20C

Antibody incubation: 120 min, 20C 1:500

(http...

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Thank you for your call today and for letting us know about the trouble with ab7747.

As we discussed, I'm sending a free of charge vial of ab18672 on the order *** which should arrive tomorrow.

Please keep me updated about the resul...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your protocol, I have looked at it in details and I would like to suggest trying the following changes to see if you start having a cytoplasmic staining rather than a nuclear staining. -check you are not overfixing (or underfixing) the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"