Anti-CD127 (phospho Y449) 抗体 (ab118527)
Key features and details
- Rabbit polyclonal to CD127 (phospho Y449)
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CD127 (phospho Y449) antibody
CD127 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD127 (phospho Y449) -
由来種
Rabbit -
特異性
ab118527 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. -
アプリケーション
適用あり: IHC-P, WB, ELISAmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Mouse CD127.
Database link: P16871-3 -
ポジティブ・コントロール
- IHC-P: Human Colon, MALT lymphoma tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118527の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.
|
|
ELISA |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 51 kDa. |
ELISA
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). -
関連疾患
Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS. -
配列類似性
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3575 Human
- Entrez Gene: 16197 Mouse
- Entrez Gene: 294797 Rat
- Omim: 146661 Human
- SwissProt: P16871 Human
- SwissProt: P16872 Mouse
- Unigene: 591742 Human
- Unigene: 635723 Human
see all -
別名
- CD 127 antibody
- CD127 antibody
- CD127 antigen antibody
see all
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab118527 は論文での使用が確認できていません。