製品の概要

  • 製品名Anti-IL7 antibody
    IL7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL7
  • アプリケーション適用あり: WB, ELISA, Neutralising, IHC-Pmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mIL-7 (mouse Interleukin-7)

  • ポジティブ・コントロール
    • IHC-P: FFPE mouse spleen tissue sections.

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプunknown
  • 軽鎖の種類unknown
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7 (using 100 µl/well antibody solution).
Neutralising Use a concentration of 0.08 - 0.1 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.50 ng/ml).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • 配列類似性Belongs to the IL-7/IL-9 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IL 7 antibody
    • IL-7 antibody
    • Il7 antibody
    • IL7_HUMAN antibody
    • Interleukin 7 antibody
    • Interleukin-7 antibody
    • LP-1 antibody
    • Lymphopoietin 1 antibody
    see all

Anti-IL7 antibody 画像

  • IHC image of IL7 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9732, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IL7 antibody (ab9732) 使用論文

This product has been referenced in:
  • Parrish YK  et al. IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow. J Immunol 182:4255-66 (2009). Neutralising ; Mouse . Read more (PubMed: 19299724) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"