製品の概要

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralising Use a concentration of 0.04 - 0.06 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (1.0 ng/ml), a concentration of 0.04 – 0.06 µg/ml of this antibody is required.

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL7 (Biotin) (ab84254).

To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84254 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-7.

ターゲット情報

  • 機能
    Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • 配列類似性
    Belongs to the IL-7/IL-9 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IL 7 antibody
    • IL-7 antibody
    • Il7 antibody
    • IL7_HUMAN antibody
    • Interleukin 7 antibody
    • Interleukin-7 antibody
    • LP-1 antibody
    • Lymphopoietin 1 antibody
    see all

画像

  • All lanes : Anti-IL7 antibody (ab9628) at 0.2 µg/ml

    Lane 1 : Recombinant human IL7 protein (ab73201) at 0.1 µg
    Lane 2 : Recombinant human IL7 protein (ab73201) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

プロトコール

参考文献

This product has been referenced in:
  • Cai YJ  et al. Up-regulation of intestinal epithelial cell derived IL-7 expression by keratinocyte growth factor through STAT1/IRF-1, IRF-2 pathway. PLoS One 8:e58647 (2013). WB ; Human . Read more (PubMed: 23554911) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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