製品の概要

  • 製品名
    Anti-IL6R antibody [B-R6]
    IL6R 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-R6] to IL6R
  • 特異性
    This antibody recognizes soluble and membrane bound IL6R.
  • アプリケーション
    適用あり: IHC-Fr, Blocking, Inhibition Assay, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    IL6R (gp80) transfected CHO cell line.

  • ポジティブ・コントロール
    • U266 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. The antibody blocks IL6 induced proliferation of XG-1 cells.
Inhibition Assay Use at an assay dependent concentration. The antibody inhibits IL6 binding to its receptor.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • 組織特異性
    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • 配列類似性
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • ドメイン
    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 翻訳後修飾
    A short soluble form may also be released from the membrane by proteolysis.
  • 細胞内局在
    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

画像

  • ab47215 staining IL6R on U266 cell line.

参考文献

This product has been referenced in:
  • Mi S  et al. Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening. Sci Rep 6:35544 (2016). ICC/IF ; Human . Read more (PubMed: 27762336) »
  • He YY  et al. Establishment of a novel cell-based assay for screening small molecule antagonists of human interleukin-6 receptor. Acta Pharmacol Sin 35:1453-62 (2014). ELISA . Read more (PubMed: 25345743) »

See all 5 Publications for this product

レビューと Q&A



I have added the protocol the lab uses to the end of this email for you. Please note that the experiment is done radioactively with Thymidine H3.

Regarding the amount of IL-6 found in the serum, 15pg/ml, it is 60 time less than the a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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