製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IL6R
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human IL6R aa 253-267.
    Database link: P08887

  • ポジティブ・コントロール
    • WB: Jurkat and 803 cell lysates. IHC-P: Human spleen tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab128008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/500. Predicted molecular weight: 52 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1 - 1/40000.

ターゲット情報

  • 機能
    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • 組織特異性
    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • 配列類似性
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • ドメイン
    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 翻訳後修飾
    A short soluble form may also be released from the membrane by proteolysis.
  • 細胞内局在
    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

画像

  • Paraffin-embedded human spleen tissue stained for IL6R with ab128008 at 2.5 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-IL6R antibody (ab128008) at 1/200 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 2 : 803 cell lysate


    Predicted band size : 52 kDa
  • ab128008 staining IL6R in Human Muller cell line MIO-M1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/400).

    See Abreview

参考文献

This product has been referenced in:
  • Zhu X  et al. IL-6R/STAT3/miR-204 feedback loop contributes to cisplatin resistance of epithelial ovarian cancer cells. Oncotarget 8:39154-39166 (2017). IHC-P, WB ; Human . Read more (PubMed: 28388577) »
  • Lin KY  et al. Genome-wide screen identified let-7c/miR-99a/miR-125b regulating tumor progression and stem-like properties in cholangiocarcinoma. Oncogene 35:3376-86 (2016). WB ; Human . Read more (PubMed: 26455324) »

See all 3 Publications for this product

レビューと Q&A

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Muller cell line MIO-M1)
Permeabilization
Yes - 0.1% TX-100
Specification
Muller cell line MIO-M1
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 02 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (HeLa cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Specification
HeLa cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% NBF
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投稿 Aug 13 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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