Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1 - 1/40000.
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
Belongs to the type I cytokine receptor family. Type 3 subfamily. Contains 1 fibronectin type-III domain. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain. The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
A short soluble form may also be released from the membrane by proteolysis.
Paraffin-embedded human spleen tissue stained for IL6R with ab128008 at 2.5 µg/ml in immunohistochemical analysis.
Western blot - Anti-IL6R antibody (ab128008)
All lanes : Anti-IL6R antibody (ab128008) at 1/200 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate Lane 2 : 803 cell lysate
Predicted band size : 52 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IL6R antibody (ab128008)This image is courtesy of an anonymous Abreview
ab128008 staining IL6R in Human Muller cell line MIO-M1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody (1/400).
Zhu X et al. IL-6R/STAT3/miR-204 feedback loop contributes to cisplatin resistance of epithelial ovarian cancer cells. Oncotarget8:39154-39166 (2017).
Read more (PubMed: 28388577) »
Lin KY et al. Genome-wide screen identified let-7c/miR-99a/miR-125b regulating tumor progression and stem-like properties in cholangiocarcinoma. Oncogene35:3376-86 (2016).
Read more (PubMed: 26455324) »