製品の概要
- 製品名IL6 overexpression 293T lysate (whole cell)
IL6 ライゼート 製品一覧 - 製品の詳細IL6 overexpression lysate
- 特記事項ab94059 is a 293T cell transfected lysate in which Human IL6 has been transiently over-expressed using a pCMV-IL6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
- アプリケーション適用あり: WBmore details
法規制情報
製品の特性
- 製品の状態Liquid
- 保存方法Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
- バッファーpH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... - 精製度Whole Cell Lysate
- 研究分野
- 背景Disease: Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. PTM: N- and O-glycosylated. Similarity: Belongs to the IL-6 superfamily.
アプリケーション
Our Abpromise guarantee covers the use of ab94059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent dilution. |
IL6 overexpression 293T lysate (whole cell) 画像
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SDS-PAGE - IL6 293T Transfected Lysate - (positive control) (ab94059)ab94059 at 15µg/lane on an SDS-PAGE gel. -
Western blot - IL6 293T Transfected Lysate - (positive control) (ab94059)All lanes : Anti-IL6 antibody (ab66231) at 1/500 dilution
Lane 1 :IL6 overexpression 293T lysate (whole cell) (ab94059) at 25 µg
Lane 2 : 293T non transfected-lysate
Secondary
Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
IL6 overexpression 293T lysate (whole cell) (ab94059) 使用論文
ab94059 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"