製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-6 (0.5 ng/ml), a concentration of 19.0-23.0 ug/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration. In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with Rabbit polyclonal to IL6 (Biotin) (ab84251) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
IHC-P Use a concentration of 0.125 µg/ml.
WB Use a concentration of 0.2 - 0.4 µg/ml. Used in conjunction with compatible secondary reagents, the detection limit for recombinant human IL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

ターゲット情報

  • 機能Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • 関連疾患Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • 配列類似性Belongs to the IL-6 superfamily.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interleukin BSF 2 antibody
    • B cell differentiation factor antibody
    • B cell stimulatory factor 2 antibody
    • B-cell stimulatory factor 2 antibody
    • BSF 2 antibody
    • BSF-2 antibody
    • BSF2 antibody
    • CDF antibody
    • CTL differentiation factor antibody
    • Hepatocyte stimulatory factor antibody
    • HGF antibody
    • HSF antibody
    • Hybridoma growth factor antibody
    • Hybridoma growth factor Interferon beta-2 antibody
    • IFN-beta-2 antibody
    • IFNB2 antibody
    • IL 6 antibody
    • IL-6 antibody
    • IL6 antibody
    • IL6_HUMAN antibody
    • Interferon beta 2 antibody
    • Interferon beta-2 antibody
    • Interleukin 6 antibody
    • Interleukin 6 (interferon beta 2) antibody
    • Interleukin BSF 2 antibody
    • Interleukin-6 antibody
    see all

Anti-IL6 antibody 画像

  • IHC image of IL6 staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9324, 1in250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-IL6 antibody (ab9324) at 1/2000 dilution

    Lane 1 : Human Spleen
    Lane 2 : Human Lung
    Lane 3 : Rat Spleen
    Lane 4 : Rat Lung

    Lysates/proteins at 20 µg per lane.

    Secondary
    800CW Goat Anti-Mouse IgG at 1/10000 dilution

    Performed under reducing conditions.

    Observed band size : 17,25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa (possible multimer).

    This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.

  • Immunohistochemical analysis of PFA-fixed frozen murine neural tissue sections, labelling IL6 with ab9324 at a dilution of 1/1000 incubated for 24 hours at 4°C in 0.1M PBST with 10% donkey serum. Permeabilization was with 0.1M PBS and 3% Triton X. Blocking was with 10% serum incubated for 1 hour at 24°C. Secondary was a mouse monoclonal Alexa Fluor® 568 conjugate at 1/1000.

    See Abreview

  • ab9324  staining IL6 in human cervical squamous cell carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of IL6 staining (ab9324) in rat primary microglial cell culture. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.3% triton X in 0.1% PBS for 1h to permeabilise the cells. The sections were then incubated with ab9324 (1:1000) overnight at +4°C, followed by Alexa 568 conjugated secondary antibody. Red staining of the cytosol was observed.

    See Abreview

Anti-IL6 antibody (ab9324) 使用論文

This product has been referenced in:
  • Yang M  et al. A Microscopic and Biomarker Evaluation of Embolic Filter Debris Collected During Carotid Artery Stenting. J Endovasc Ther N/A:N/A (2016). Read more (PubMed: 26839124) »
  • Yang N  et al. TNF-a receptor antagonist attenuates isoflurane-induced cognitive impairment in aged rats. Exp Ther Med 12:463-468 (2016). WB ; Rat . Read more (PubMed: 27347079) »

See all 8 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Muller Cell Line MIO-M1)
Permeabilization Yes - 0.1% TritonX-100
Specification Muller Cell Line MIO-M1
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 01 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Aug 20 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Tris, 1mM EDTA pH 9
Sample Monkey Tissue sections (retina)
Specification retina
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 10 2015

Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-15% SDS-PAGE)
Sample Monkey Tissue lysate - whole (macaque retina)
Specification macaque retina
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 10 2015

All of these antibodies recognise cytokines which are present in inflammed tissue therefore any inflammed tissue would be a good positive control.

In our catalogue, we have several cancer slides which would be appropriate:
tonsil: (ab514...

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Any products that require an MSDS form will have a link on the product's online datasheet to the data. It will be a PDF document link labeled 'SDS'. Please see attached image for example of what to look for on the datasheet. If no 'SDS' link ...

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DISCOUNT CODES:
***

Expiration date: November 26, 2012

These codes will each give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include this code in...

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Thank you very much for your call today and for your interest in our IL6 and IL8 antibodies.

I've looked over the rest of our IL6 antibodies, and we unfortunately don't have any that Istrongly expect to cross-react with rabbit samples. The mo...

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Thank you for contacting us.

The anti-IL6 antibody ab9324 is a mouse monoclonal IgG2a. Therefore, you will need to use a mouse monoclonal IgG2a isotype control.

Therefore, you should use the mouse control IgG2a ab18413 and NOT the ...

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Thank you for contacting Abcam regarding ab9324.

The clone number for this antibody is 1.2-2B11-2G10.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"