Anti-IL-4 抗体 [EPR1118Y] (ab62351)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1118Y] to IL-4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-4 antibody [EPR1118Y]
IL-4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1118Y] to IL-4 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human IL-4 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human tonsil lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1118Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 17 kDa.
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ICC/IF |
1/500.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Predicted molecular weight: 17 kDa. |
ICC/IF
1/500. |
ターゲット情報
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機能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
関連疾患
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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別名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
画像
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Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling IL4 with unpurified ab62351 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) labelling IL4 with purified ab62351 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
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Anti-IL-4 antibody [EPR1118Y] (ab62351) at 1/2000 dilution + Human tonsil lysate at 10 µg
Secondary
goat anti rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab62351 は 9 報の論文で使用されています。
- Liu G et al. A novel inflammation-associated prognostic signature for clear cell renal cell carcinoma. Oncol Lett 24:307 (2022). PubMed: 35949606
- Almeida AF et al. Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles. Int J Mol Sci 23:N/A (2022). PubMed: 36499452
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Becares N et al. Immune Regulatory Mediators in Plasma from Patients With Acute Decompensation Are Associated With 3-Month Mortality. Clin Gastroenterol Hepatol 18:1207-1215.e6 (2020). PubMed: 31446184
- Granier C et al. Formation of somatosensory detour circuits mediates functional recovery following dorsal column injury. Sci Rep 10:10953 (2020). PubMed: 32616790
- Tao S et al. Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway. J Exp Clin Cancer Res 39:191 (2020). PubMed: 32943090
- Deng S et al. Relationship Between Pregnancy and Acute Disseminated Encephalomyelitis: A Single-Case Study. Front Immunol 11:609476 (2020). PubMed: 33597947
- Ganieva U et al. Involvement of Transcription Factor 21 in the Pathogenesis of Fibrosis in Endometriosis. Am J Pathol 190:145-157 (2020). PubMed: 31610174
- Xie N et al. miR-320a upregulation contributes to the development of preeclampsia by inhibiting the growth and invasion of trophoblast cells by targeting interleukin 4. Mol Med Rep 20:3256-3264 (2019). PubMed: 31432141