製品の概要

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
ELISA Use at an assay dependent concentration. To detect hIL-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required.
IHC-P Use a concentration of 0.25 µg/ml.
Sandwich ELISA Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair.

ターゲット情報

  • 機能
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  • 関連疾患
    Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 配列類似性
    Belongs to the IL-4/IL-13 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell growth factor 1 antibody
    • B cell IgG differentiation factor antibody
    • B Cell Stimulatory Factor 1 antibody
    • B-cell stimulatory factor 1 antibody
    • BCGF 1 antibody
    • BCGF1 antibody
    • Binetrakin antibody
    • BSF-1 antibody
    • BSF1 antibody
    • IGG1 induction factor antibody
    • IL 4 antibody
    • IL-4 antibody
    • IL4 antibody
    • IL4_HUMAN antibody
    • Il4e12 antibody
    • Interleukin 4 antibody
    • Interleukin 4 variant 2 antibody
    • Interleukin 4, isoform 1 antibody
    • Interleukin-4 antibody
    • Lymphocyte stimulatory factor 1 antibody
    • MGC79402 antibody
    • Pitrakinra antibody
    see all

Anti-IL4 antibody 画像

  • ab9622 staining IL4 in human breast invasive ductal carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. Additional protein blocks were used in conjuction with secondary reagents.

  • Anti-IL4 antibody (ab9622) at 0.2 µg/ml + Recombinant human IL4 protein (ab73186) at 0.0001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

Anti-IL4 antibody (ab9622) 使用論文

This product has been referenced in:
  • Furukawa S  et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep 7:42413 (2017). IHC ; Human . Read more (PubMed: 28205524) »
  • Naeem AS  et al. A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis. J Allergy Clin Immunol 139:1228-1241 (2017). Read more (PubMed: 27913303) »

See all 15 Publications for this product

Product Wall

A good alternative to tonsilis spleen and maybe also tissue from the digestive tract, for instance colon or small intestine.

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor)
Specification
Tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Username

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Verified customer

投稿 Jan 16 2012

DISCOUNT CODE:*** Expiration date: May 13, 2012 I am very pleased to hear you would like to test ab9622 in mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ab9622 in mo...

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Normal human spleen and lymph node should be a good positive controls. The antigen retrieval is mentioned under the IHC-P image on the datasheet- sodium citrate buffer (pH 6.0)- and other main specifications of the protocol used.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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