Rabbit polyclonal to IL3RB (phospho Y593)
ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.
Synthetic phosphopeptide derived from human IL3RB around the phosphorylation site of tyrosine 593 (G-P-Y
Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Information by UniProt
/granulocyte-macrophage colony-stimulating factor receptor, beta antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RB (phospho Y593) antibody (ab53695)
ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Western blot - Anti-IL3RB (phospho Y593) antibody (ab53695)
All lanes : Anti-IL3RB (phospho Y593) antibody (ab53695) Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide Predicted band size: 97 kDa
has not yet been referenced specifically in any publications.
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