製品の概要

  • 製品名Anti-IL36 beta antibody
    IL36 beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL36 beta
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human IL36 beta aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9NZH7

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Tonsil tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).

ターゲット情報

  • 組織特異性Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
  • 配列類似性Belongs to the IL-1 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2310043N20Rik antibody
    • Family of interleukin 1 eta antibody
    • FIL1 (ETA) antibody
    • FIL1 antibody
    • FIL1 eta antibody
    • FIL1H antibody
    • FILI-(ETA) antibody
    • IL 1F8 (FIL1 eta antibody
    • IL 1F8 antibody
    • IL 1H2 antibody
    • IL-1 eta antibody
    • IL-1F8 antibody
    • IL-1H2 antibody
    • IL1 ETA antibody
    • IL1F8 (Canonical product IL 1F8a) antibody
    • IL1F8 antibody
    • IL1F8_HUMAN antibody
    • IL1H2 antibody
    • IL36B antibody
    • Interleukin 1 family, member 8 (eta) antibody
    • Interleukin 1 family, member 8 antibody
    • Interleukin 1 homolog 2 antibody
    • Interleukin 1 Superfamily e antibody
    • Interleukin 36 beta antibody
    • Interleukin-1 eta antibody
    • Interleukin-1 family member 8 antibody
    • Interleukin-1 homolog 2 antibody
    • MGC126880 antibody
    • MGC126882 antibody
    • OTTMUSP00000012797 antibody
    • RP23-176J12.1 antibody
    see all

Anti-IL36 beta antibody 画像

  • All lanes : Anti-IL36 beta antibody (ab116280) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Tonsil (Human) Tissue Lysate
    Lane 2 : Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL36 beta antibody (ab116280) 使用論文

ab116280 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab116280.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"