Rabbit polyclonal to IL36 beta
Macaque monkey, Gorilla
Synthetic peptide corresponding to Human IL36 beta aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q9NZH7
This antibody gave a positive signal in Human Tonsil tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).
Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
Belongs to the IL-1 family.
Information by UniProt
Family of interleukin 1 eta antibody
FIL1 (ETA) antibody
Western blot - Anti-IL36 beta antibody (ab116280)
All lanes :
Anti-IL36 beta antibody (ab116280) at 1 µg/ml (Milk blocking 3%)
Lane 1 :
Tonsil (Human) Tissue Lysate
Lane 2 :
Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Donkey Anti-Rabbit IgG H&L preadsorbed (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
20 kDa (
why is the actual band size different from the predicted?
Additional bands at:
37 kDa (possible non-specific binding)
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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