製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IL36 alpha
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human IL36 alpha aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9UHA7

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Adult and Fetal Brain tissue lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).

ターゲット情報

  • 組織特異性
    Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.
  • 配列類似性
    Belongs to the IL-1 family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FIL1 antibody
    • FIL1 epsilon antibody
    • FIL1(EPSILON) antibody
    • FIL1E antibody
    • IL 1 epsilon antibody
    • IL 1F6 antibody
    • IL 1H1 antibody
    • IL-1 epsilon antibody
    • IL-1F6 antibody
    • IL1E antibody
    • IL1F6 antibody
    • IL1F6_HUMAN antibody
    • IL1RP2 antibody
    • IL36A antibody
    • Interleukin 1 epsilon antibody
    • Interleukin 1 family member 6 (epsilon) antibody
    • Interleukin 1 family member 6 antibody
    • Interleukin 36 alpha antibody
    • Interleukin-1 epsilon antibody
    • Interleukin-1 family member 6 antibody
    • MGC129552 antibody
    • MGC129553 antibody
    • MGC151479 antibody
    • MGC151481 antibody
    • OTTMUSP00000012798 antibody
    • RP23-176J12.4 antibody
    see all

画像

  • All lanes : Anti-IL36 alpha antibody (ab116279) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/ml
    Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116279 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

プロトコール

参考文献

ab116279 has not yet been referenced specifically in any publications.

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