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Our Abpromise guarantee covers the use of ab110535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use a concentration of 0.2 - 0.4 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL33 (1.0 ng/ml).|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. The detection limit for recombinant hIL33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. ab110535, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL33 (Biotin) (ab84249). ab110535, in conjunction with ab84249 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.|
ab110535 has not yet been referenced specifically in any publications.