Anti-IL31 抗体 (ab102750)

製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab102750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-IL31 antibody 画像

  • Anti-IL31 antibody (ab102750) at 1/100 dilution + Mouse spleen tissue lysates at 35 µg

    Predicted band size : 18 kDa
  • ab102750, at 1/50 dilution, staining IL31 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
  • ab102750 staining IL31 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab102750 at a 1/75 dilution for 14 hours at 4°C. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    Green : ab102750
    Red : pgp 9.5
    Yellow : merge

    See Abreview

Anti-IL31 antibody (ab102750) 使用論文

ab102750 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization Yes - 0.1% Tween 20
Specification Tonsil
Blocking step BioGenex Power Block 10 mins then BioGenex NGS Protein Block 30 mins as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative Formaldehyde
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Abcam user community

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投稿 Sep 19 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Cell (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
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Abcam user community

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投稿 Sep 12 2015


Das Labor hat mir mitgeteilt, dass der WB unter reduzierten und denaturierten Bedingungen durchgefuehrt wurde.

Das genaue Protocol ist wie folgt:

Western Blotting Protocol

A. Preparation of cell lysates

1. Coll...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (PBMCs)
Loading amount 500000 cells
Specification PBMCs
Treatment see below (Additional notes)
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

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投稿 Jul 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization Yes - 0,1% Saponin / PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 13 2011

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Skin)
Specification Skin
Fixative Acetone
Permeabilization Yes - Saponin 0.1% PBS, 30 minutes
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Ms. Bryony Stott

Verified customer

投稿 May 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"