Anti-IL-27-A 抗体 (ab118910)
Key features and details
- Rabbit polyclonal to IL-27-A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-27-A antibody
IL-27-A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-27-A -
由来種
Rabbit -
特異性
ab118910 recognize IL27-A (NP_663634). -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human IL-27-A (internal sequence) conjugated to keyhole limpet haemocyanin. Corresponds to 14 amino acids near the center of Human IL27-A.
Database link: NP_663634 -
ポジティブ・コントロール
- Daudi lysate, Human Spleen Neutrophils tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 27 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 27 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. -
組織特異性
Expressed in monocytes and in placenta. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Secreted. Does not seem to be secreted without coexpression of EBI3. - Information by UniProt
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参照データベース
- Entrez Gene: 246778 Human
- Entrez Gene: 246779 Mouse
- Omim: 608273 Human
- SwissProt: Q8NEV9 Human
- SwissProt: Q8K3I6 Mouse
- Unigene: 528111 Human
- Unigene: 222632 Mouse
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別名
- EBI3 antibody
- IL 27 antibody
- IL 27 p28 subunit antibody
see all
画像
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Human Spleen, Neutrophils (formalin-fixed, paraffin-embedded) stained with ab118910 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Lane 1 : Anti-IL-27-A antibody (ab118910) at 2 µg/ml
Lane 2 : Anti-IL-27-A antibody (ab118910) at 4 µg/ml
All lanes : Daudi lysate
Predicted band size: 27 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab118910 は 2 報の論文で使用されています。
- Di Carlo E Decoding the Role of Interleukin-30 in the Crosstalk Between Cancer and Myeloid Cells. Cells 9:N/A (2020). PubMed: 32143355
- Di Meo S et al. Interleukin-30 expression in prostate cancer and its draining lymph nodes correlates with advanced grade and stage. Clin Cancer Res 20:585-94 (2014). Human . PubMed: 24277453