Anti-IL23 抗体 [B-Z23] (ab84471)

製品の概要

  • 製品名Anti-IL23 antibody [B-Z23]
    IL23 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-Z23] to IL23
  • 特異性Recognizes both recombinant and natural human IL23, p19 sub-unit.
  • アプリケーション適用あり: Neutralising, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant Human IL23 (sub-unit p19)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Ion Exchange Chromatography
  • 特記事項(精製)Sterile-filtered through 0.22 µm and treated to remove endotoxins.
  • ポリ/モノモノクローナル
  • クローン名B-Z23
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Neutralising Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 組織特異性Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 配列類似性Belongs to the IL-6 superfamily.
  • 発生段階Expressed by newborns dendritic cells.
  • 細胞内局在Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

Anti-IL23 antibody [B-Z23] 画像

  • ab84472 used in Neutralisation.
    A = untreated
    B = treated with IL-2 (10ng/ml) + IL-23 (10ng)
    C = treated with IL-2 (10ng/ml) + IL-23 (50ng/ml)
    D = treated with anti-IL-23 (5µg/ml) + IL-2 + IL-23 (50ng/ml)
    Human monocytes were fixed in methanol, permeabilized using 0.1% Saponin/ PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab84471 at 5µg/ml for 48 hours at 37°C.

    Staining: IL-17 (red) GFP (green).

    We have examined the effect of IL-23 and in combination with anti-IL-23 on IL-17 production in monocytes. IL-23 leads to increased IL-17 secretion (Figure B and C) and this effect was abolished with anti-IL-23 (Figure D). IL-2 is part of our culture medium for monocytes. IL-2 stimulation serves as a basis to keep the cell alive. Thus almost every cytokine that we used is used in combination with IL-2. Because it is part of the medium, IL-2 can be omitted when specifying the stimulation. The cells were stained with the GFP (Green Fluorescent Protein), just to make them visible. We opted for GFP, because the cells are less affected by GFP as by dyes.The red color represents the IL-17 which was the target in this experiment. The secretion of IL-17 was tested with and without IL-23 and in combination with anti-IL-23. If the green of the GFP interferes, all images can be shown without the green channel (red only).

    See Abreview

Anti-IL23 antibody [B-Z23] (ab84471) 使用論文

ab84471 has not yet been referenced specifically in any publications.

Product Wall

The lab has provide me with the neutralization protocol used by them. The peptides and reagents are all listed on this. The test is done on murine cells for human cytokine, the mIL-23 and hIL-23 are closed and cross-reacted on cells from mice or human....

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Thank you for contacting us.

The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more spe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Monocytes)
Specification Monocytes
Fixative Methanol
Permeabilization Yes - 0,1% Saponin/PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Feb 15 2012

Thank you for contacting us. I have attached the protocol that we received from the laboratory that tested this IL23 antibody for neutralization. The assay measures the amount of IL17 that is produced by mouse splenocytes in response to IL23 and IL2...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"