製品の概要

  • 製品名Anti-IL23 antibody
    IL23 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL23
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 16 amino acid synthetic peptide conjugated to KLH, corresponding to the C-terminus of Human IL23.

  • ポジティブ・コントロール
    • Human Kidney, Mouse brain, Mouse kidney and Mouse liver tissues

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 組織特異性Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 配列類似性Belongs to the IL-6 superfamily.
  • 発生段階Expressed by newborns dendritic cells.
  • 細胞内局在Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

Anti-IL23 antibody 画像

  • All lanes : Anti-IL23 antibody (ab115759) at 1 µg/ml

    Lane 1 : Raji cell lysate
    Lane 2 : Raji cell lysate


    Predicted band size : 21 kDa

    Western blot of IL-23 in Raji cell lysate with ab115759 at 1 ug/ml in the (1) absence and (2) presence of blocking peptide.

  • Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Brain (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Liver (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-IL23 antibody (ab115759) 使用論文

This product has been referenced in:
  • Szkandera J  et al. A functional germline variant in GLI1 implicates hedgehog signaling in clinical outcome of stage II and III colon carcinoma patients. Clin Cancer Res 20:1687-97 (2014). Human . Read more (PubMed: 24470513) »
  • Helbling M  et al. Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients. Oncotarget 5:4671-82 (2014). IHC . Read more (PubMed: 25015728) »

See all 2 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Ich kann Ihnen ab115759 (Anti-IL23 antibody) empfehlen.

http://www.abcam.com/index.html?datasheet=115759 (or use the following: http://www.abcam.com/index.html?datasheet=115759).

Dieser A...

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Thank you for contacting us.

The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more spe...

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Thank you for contacting us.

The C-terminal sequence of IL23A protein is quite homologous between Bovina and Human. http://www.uniprot.org/blast/uniprot/2012102343Z34R5CGA?alignment=25

It seems that the antibody e.g. ab115759; http://...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

/Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frageboge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"