製品の概要

  • 製品名
    Anti-IL22 Receptor Alpha antibody
    IL22 Receptor Alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL22 Receptor Alpha
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    QSSNFENILTWDSGPE

    , corresponding to amino acids 31-46 of Human IL-22 Receptor.

  • ポジティブ・コントロール
    • WB: HepG2 whole cell lysate. IHC-P: Rat kidney tissue. ICC/IF: HepG2 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with IL22 Receptor Alpha peptide (ab6229).
IHC-P Use a concentration of 5 µg/ml. PubMed: 19535621
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-IL22 Receptor Alpha antibody 画像

  • Lane 1 : Anti-IL22 Receptor Alpha antibody (ab5984) at 0.5 µg/ml
    Lane 2 : Anti-IL22 Receptor Alpha antibody (ab5984) at 1 µg/ml

    Lane 1 : HepG2 cell lysates
    Lane 2 : HepG2 cell lysates
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL22 Receptor Alpha with ab5984 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL22 Receptor Alpha with ab5984 at 20µg/ml.

  • Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling IL22 Receptor Alpha with ab5984 at 10µg/ml.

  • Immunocytochemistry of IL-22R in HepG2 cells with IL-22R antibody at 10 µg/ml.
  • Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml. Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml.

Anti-IL22 Receptor Alpha antibody (ab5984) 使用論文

This product has been referenced in:
  • Andoh A  et al. Expression of IL-24, an activator of the JAK1/STAT3/SOCS3 cascade, is enhanced in inflammatory bowel disease. J Immunol 183:687-95 (2009). IHC-P ; Human . Read more (PubMed: 19535621) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

投稿 Jul 05 2016

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Lung)
Specification
Lung
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 25 2014

Application
Flow Cytometry
Fixation
Surface staining
Permeabilization
No
Sample
Human Cell (Epidermal keratinocytes)
Specification
Epidermal keratinocytes
Gating Strategy
Human epidermal keratinocytes
Preparation
Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: Biolegend cell staining buffer
Username

Abcam user community

Verified customer

投稿 Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録