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Our Abpromise guarantee covers the use of ab109819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. The detection limit for recombinant Mouse IL22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. ab109819 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.|
|Neutralising||Use a concentration of 2 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL22 (50.0 ng/ml).|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL22 (Biotin) (ab84225). ab109819 in conjunction with ab84225 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL22.|