The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.25-0.5µg for 105-8 cells.
in a final volume of 100 µL
ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
This is a receptor for interleukin-21.
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
Flow cytometry image showing staining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a ? Isotype Control Biotin (open histogram) or 0.25 µg of ab95712 (filled histogram) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
Vosshenrich CA et al. Roles for common cytokine receptor gamma-chain-dependent cytokines in the generation, differentiation, and maturation of NK cell precursors and peripheral NK cells in vivo. J Immunol174:1213-21 (2005).
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