The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µl is recommended.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
This is a receptor for interleukin-21.
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.
Belongs to the type I cytokine receptor family. Type 4 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.
Flow cytometry staining of 2-day Anti-Mouse CD3e stimulated BALB/c splenocytes with Anti-Mouse CD4 APC and 0.25 µg of Rat IgG2a ? Isotype Control (left) or 0.25 µg of ab95711 (right) followed by Anti-Rat IgG Biotin and then Streptavidin PE. Total viable cells were used for analysis.