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TCPSCDSYEKKPPKE, corresponding to amino acids 121-135 of Human IL-21.
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Our Abpromise guarantee covers the use of ab5978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.Can be blocked with IL21 peptide (ab6680).|
ab5978 staining IL21 in mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/8000 in 10% normal goat serum) for 10 hours. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/350) was used as the secondary antibody.
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