Anti-IL2 Receptor alpha 抗体 [MEM-181] (ab8235)

製品の概要

  • 製品名Anti-IL2 Receptor alpha antibody [MEM-181]
    IL2 Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-181] to IL2 Receptor alpha
  • 特異性CD25 antigen (human).
  • アプリケーション適用あり: Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Tissue/ cell preparation (Human). (PHA-activated peripheral blood leucocytes).

  • エピトープextracellular

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/1000.

ターゲット情報

  • 機能Receptor for interleukin-2.
  • 関連疾患Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 配列類似性Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Anti-IL2 Receptor alpha antibody [MEM-181] 画像

  • Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-IL2 Receptor alpha antibody [MEM-181] (ab8235) 使用論文

This product has been referenced in:
  • Brusés JL Cell surface localization of a3ß4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility. PLoS One 8:e62435 (2013). ICC/IF ; Human . Read more (PubMed: 23626818) »
  • Nielsen MS  et al. Different motifs regulate trafficking of SorCS1 isoforms. Traffic 9:980-94 (2008). ICC/IF ; Mouse . Read more (PubMed: 18315530) »

See all 2 Publications for this product

Product Wall

The antibody ab8232 is unconjugated and so you would need to use a secondary antibody for detection. If you tell me what techniques you are using I would be happy to make some suggestions about suitable secondary antibodies. Please let me know if ther...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK293 tranfected with chimeric receptor construct)
Specification HEK293 tranfected with chimeric receptor construct
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
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Abcam user community

Verified customer

投稿 Jul 17 2008

I can confirm that this product does not required pemeabilisation when it was tested in FACS as the recognized epitope is extracellular. Please do not hesitate to contact us if you require further information.

Thank you for your enquiry. I ran a search for a neutralising antibody directed against the IL2 Receptor alpha and unfortunately could find any results. This does not mean that the ab8235 will not work in blocking IL2, it just means that we have not te...

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Thank you for your message. We are very sorry but there was a discrepancy on our datasheet. We would like to confirm that this antibody has not been tested and characterised for Western blot application. Thank you for your feedback; it is very ...

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Our datasheet currently states that the antibody has not yet been tested in WB and I apologise if it previously did; I have checked with our source and they confirmed they have not tested it yet. Should you wish a refund can you please give me your...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"