Anti-IL2 Receptor alpha 抗体 [IL2R.1] (ab9496)

製品の概要

  • 製品名
    Anti-IL2 Receptor alpha antibody [IL2R.1]
    IL2 Receptor alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [IL2R.1] to IL2 Receptor alpha
  • 特異性
    This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    BALB/C mice were injected with recombinant human interleukin-2 receptor.

  • ポジティブ・コントロール
    • Tonsil.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Receptor for interleukin-2.
  • 関連疾患
    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 配列類似性
    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

画像

  • ab9496 - immunohistochemistry

    Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.

  • Overlay histogram showing Jurkat cells stained with ab9496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9496, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH
Sample
Human Tissue sections (Malignant Melanoma)
Specification
Malignant Melanoma
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 06 2015

Thank you for contacting Abcam.

I am sorry that the vial you received was not kept cold. However, we do have data that provides supporting evidence that the antibody should still function properly. I have attached that data.

I hope th...

Read More

Use heat mediated antigen retrieval via pressure cooker or microwave. Please see the antigen retrieval protocol linked on the right hand side of the Abcam homepage. Use an ABC amplification system (also linked on the Abcam homepage).

This antibody has never been tested in flow cytometry, therefore we can't guarantee results. However, being as this antibody recognises a cell surface marker, I would assume it would work in flow but as previously stated I can't be certain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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