The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: 1/10000 will readily detect 5 ng of protein. ELISA: Use at an assay dependent dilution. Neut: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 17 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.