Anti-IL-1RAPL1 抗体 (ab117480)
Key features and details
- Rabbit polyclonal to IL-1RAPL1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-IL-1RAPL1 antibody
IL-1RAPL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-1RAPL1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Chicken, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Mouse Brain and Mouse Hippocampus tissue lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117480の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/250. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).
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特記事項 |
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WB
1/250. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa). |
ターゲット情報
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機能
May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. May activate the MAP kinase JNK. -
組織特異性
Detected at low levels in heart, skeletal muscle, ovary, skin, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Detected at very low levels in tonsil, prostate, testis, small intestine, placenta, colon and fetal liver. -
関連疾患
Defects in IL1RAPL1 are the cause of mental retardation X-linked type 21 (MRX21) [MIM:300143]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs. -
配列類似性
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
細胞内局在
Cell membrane. Cytoplasm. May localize to the cell body and growth cones of dendrite-like processes. - Information by UniProt
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参照データベース
- Entrez Gene: 11141 Human
- Entrez Gene: 331461 Mouse
- Omim: 300206 Human
- SwissProt: Q9NZN1 Human
- SwissProt: P59823 Mouse
- Unigene: 658912 Human
- Unigene: 121680 Mouse
- Unigene: 426143 Mouse
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別名
- IL 1 RAPL 1 antibody
- IL 1RAPL 1 antibody
- IL-1-RAPL-1 antibody
see all
画像
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All lanes : Anti-IL-1RAPL1 antibody (ab117480) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1/250 dilution
Lane 4 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1/250 dilution
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 103 kDa (possible non-specific binding), 32 kDa (possible non-specific binding)
Exposure time: 12 minutesIL1RAPL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab117480 は論文での使用が確認できていません。