製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IL1RAP
  • 特異性
    It has nocross activity to other members in the IL-1 receptor family.
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human IL1RAP aa 549-569.
    Sequence:

    KKSPRRSSSDEQGLSYSSLKN


    (Peptide available as ab6105)

  • ポジティブ・コントロール
    • HeLa whole cell lysate
  • 特記事項


    The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associatedkinase (IRAK), which mediates activation of NF-kBinducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 18765825
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa.Can be blocked with IL1RAP peptide (ab6105).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Coreceptor with IL1R1. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
  • 組織特異性
    Detected in liver, skin, placenta, thymus and lung.
  • 配列類似性
    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C3orf13 antibody
    • FLJ37788 antibody
    • IL 1 receptor accessory protein antibody
    • IL 1R 3 antibody
    • IL 1R3 antibody
    • IL 1RAcP antibody
    • IL 1RAP antibody
    • IL-1 receptor accessory protein antibody
    • IL-1R-3 antibody
    • IL-1R3 antibody
    • IL-1RAcP antibody
    • IL1 receptor accessory protein antibody
    • IL1AP_HUMAN antibody
    • IL1R3 antibody
    • IL1RAcP antibody
    • Il1rap antibody
    • Interleukin 1 Accessory Protein antibody
    • interleukin 1 receptor 3 antibody
    • Interleukin 1 receptor accessory protein antibody
    • interleukin 1 receptor accessory protein beta antibody
    • Interleukin-1 receptor 3 antibody
    • Interleukin-1 receptor accessory protein antibody
    see all

画像

  • Western Blot of K562 cell lysate labeling IL1RAP with Anti-IL1RAP antibody (ab8109) at 1µg/ml.
  • Anti-IL1RAP antibody (ab8109) at 1/500 dilution + Hela whole cell lysate

    Observed band size : 66 kDa (why is the actual band size different from the predicted?)
  • ab8109 at 5µg/ml staining IL1RAP in Hela cells by ICC/IF

参考文献

This product has been referenced in:
  • Balagurunathan Y  et al. Gene expression profiling-based identification of cell-surface targets for developing multimeric ligands in pancreatic cancer. Mol Cancer Ther 7:3071-80 (2008). ICC/IF, IHC-P ; Human . Read more (PubMed: 18765825) »

See 1 Publication for this product

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