製品の概要

  • 製品名Anti-IL18R1 antibody
    IL18R1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL18R1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human IL18R1 aa 500 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q13478

  • ポジティブ・コントロール
    • This antibody gave a positive signal in both Human Spleen and Lung tissue lysates as well as Hodgkin's Lymphoma (Human) Whole Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Receptor for interleukin 18 (IL-18). Binding to the agonist leads to the activation of NF-kappa-B.
  • 組織特異性Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.
  • 配列類似性Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD218 antigen-like family member A antibody
    • CD218a antibody
    • CDw218a antibody
    • CDw218a antigen antibody
    • IL 1Rrp antibody
    • IL-18R-1 antibody
    • IL-18R1 antibody
    • IL-1Rrp antibody
    • IL1 receptor related protein antibody
    • IL1 receptor-related protein antibody
    • IL18R_HUMAN antibody
    • IL18R1 antibody
    • IL18RA antibody
    • Il18ralpha antibody
    • IL1R-rp antibody
    • IL1RRP antibody
    • Interleukin 18 receptor 1 antibody
    • Interleukin 18 receptor, alpha chain antibody
    • Interleukin-18 receptor 1 antibody
    see all

Anti-IL18R1 antibody 画像

  • IHC image of IL18R1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117432, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-IL18R1 antibody (ab117432) at 1 µg/ml + Hodgkin's Lymphoma (Human) Whole Cell Lysate - tumor tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    The band observed at 60 kDa could potentially be a cleaved form of IL18R1 due to the presence of an 18 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117432 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • All lanes : Anti-IL18R1 antibody (ab117432) at 1 µg/ml

    Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    The band observed at 60 kDa could potentially be a cleaved form of IL18R1 due to the presence of an 18 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117432 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL18R1 antibody (ab117432) 使用論文

ab117432 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"