Rabbit polyclonal to IL17D
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17D.
(Peptide available as
This antibody gave a positive signal in the following lysates:
Human Thymus Tissue; Jurkat Whole Cell
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells.
Expressed preferentially in adipose, skeletal muscle and CNS.
Belongs to the IL-17 family.
Information by UniProt
IL 17D antibody
IL 22 antibody
IL 27 antibody
Western blot - IL17D antibody (ab77185)
All lanes :
Anti-IL17D antibody (ab77185) at 1 µg/ml
Lane 1 :
Human thymus tissue lysate - total protein (
Lane 2 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
27 kDa (
why is the actual band size different from the predicted?
Additional bands at:
15 kDa. We are unsure as to the identity of these extra bands.
IL17D contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IL17D antibody (ab77185)
IHC image of 77185 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77185, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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