Anti-IL-13 receptor alpha 1 抗体 [GM1E7] (ab39418)
Key features and details
- Mouse monoclonal [GM1E7] to IL-13 receptor alpha 1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-IL-13 receptor alpha 1 antibody [GM1E7]
IL-13 receptor alpha 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [GM1E7] to IL-13 receptor alpha 1 -
由来種
Mouse -
特異性
The antibody recognises IL-13 Receptor 1 alpha transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes. -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Other Immunogen Type corresponding to Human IL-13 receptor alpha 1. Genetic immunization with cDNA encoding human IL13-Rα1 (extracellular domain)
Database link: P78552 -
ポジティブ・コントロール
- ICC/IF: CHO cells transiently transfected with an expression vector encoding IL13-R. Flow Cyt: IL13-R transfected BOSC23 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
GM1E7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39418の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | ||
ICC/IF |
特記事項 |
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Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity. -
組織特異性
Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Membrane. - Information by UniProt
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別名
- bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
- Cancer/testis antigen 19 antibody
- CD213a1 antibody
see all
画像
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Flow cytometric analysis of BOSC23 cells using ab39418. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ab39418 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.
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Immunofluorescence staining of CHO cells using ab39418 at 1 µg/106 cells. CHO cells were transiently transfected with an expression vector encoding IL13-R. Binding of ab39418 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
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Specificity testing of ab39418. BOSC cells were transiently transfected with an expression vector encoding either the extracellular domain of IL-13Rα1 or an irrelevant protein. Expression of the constructs was tested with an anti-tag monoclonal antibody (B, green curves), an irrelevant monoclonal antibody served as negative control (bluecurves). For specificity testing (A), purified ab39418 was tested on both the IL-13Rα1 (red curve) and the irrelevant protein trans-fectants (green curve). A posi-tive signal was obtained only with IL-13Rα1 transfected cells.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab39418 は 1 報の論文で使用されています。
- Trüb M et al. Fibroblast activation protein-targeted-4-1BB ligand agonist amplifies effector functions of intratumoral T cells in human cancer. J Immunother Cancer 8:N/A (2020). PubMed: 32616554