Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
Our Abpromise guarantee covers the use of ab9576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
ELISA | Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution). | |
Neutralising | Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml). | |
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
15% denaturing gel. Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.
Calibration curve of a sandwich assay for IL13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.
ab9576 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"