製品の概要
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製品名Anti-IL13 antibody
IL13 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IL13
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アプリケーション適用あり: WB, ELISA, Neutralising, IHC-Pmore details
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種交差性交差種: Human
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免疫原
Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
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ポジティブ･コントロール
- IHC-P: human testis tissue sections
製品の特性
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製品の状態Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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バッファーPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプunknown
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軽鎖の種類unknown
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab9576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| ELISA | Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution). | |
| Neutralising | Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
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関連疾患Allergic rhinitis
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配列類似性Belongs to the IL-4/IL-13 family.
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細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
- Unigene: 845 Human
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別名
- Allergic rhinitis antibody
- ALRH antibody
- BHR1 antibody
see all
Anti-IL13 antibody 画像
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Western blot - IL13 antibody (ab9576)This image is courtesy of an Abreview submitted by Miss Fenny ChongAll lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilution
Lane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µg
Lane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µg
Lane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µg
Lane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µg
Lane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µg
Lane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µg
Lane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µg
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique
Performed under non-reducing conditions.
Observed band size : 10 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa (possible dimer).
Exposure time : 5 minutesThis image is courtesy of an Abreview submitted by Miss Fenny Chong
15% denaturing gel. Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL13 antibody (ab9576)IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Sandwich ELISA - Anti-IL13 antibody (ab9576)Calibration curve of a sandwich assay for IL13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.
Anti-IL13 antibody (ab9576) 使用論文
ab9576 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"