製品の概要

  • 製品名Anti-IL13 antibody
    IL13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL13
  • アプリケーション適用あり: WB, ELISA, Neutralising, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)

  • ポジティブ・コントロール
    • IHC-P: human testis tissue sections

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 関連疾患Allergic rhinitis
  • 配列類似性Belongs to the IL-4/IL-13 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

Anti-IL13 antibody 画像

  • All lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilution

    Lane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µg
    Lane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µg
    Lane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µg
    Lane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µg
    Lane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µg
    Lane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µg
    Lane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible dimer).

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Miss Fenny Chong

    See Abreview

  • IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IL13 antibody (ab9576) 使用論文

ab9576 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization No
Specification Lung
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (E.coli expressed commercial protein)
Loading amount 0.3 µg
Specification E.coli expressed commercial protein
Gel Running Conditions Non-reduced Denaturing (15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Miss. Fenny Chong

Verified customer

投稿 Mar 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"