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Our Abpromise guarantee covers the use of ab109815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL13.|
|Neutralising||Use a concentration of 0.08 - 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL13 (10.0 ng/ml)|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL13 (Biotin) (ab83162). Using ab83162 as the detection antibody and ab109815 as the capture antibody, the detection limit for recombinant Mouse IL13 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Mouse IL13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|