Anti-IL-11RA 抗体 [EPR5446] (ab125015)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5446] to IL-11RA
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, Indirect ELISA
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-IL-11RA antibody [EPR5446]
IL-11RA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5446] to IL-11RA -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, Indirect ELISAmore details
適用なし: IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human IL-11RA aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q14626 -
ポジティブ・コントロール
- WB: 293T, Saos2, A673, PC3, HuT78, K562, and TF1 cell lysates; RAW264.7, WEHI-231, and M1 mouse cell lysates. Mouse spleen, testis, thymus, and lymph node tissue lysates; IHC-P: Human kidney tissue; Flow Cyt (intra): K562 cells; ICC/IF: K562 cells;
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.78 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5446 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125015の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use in 1/100 - 1/1000 |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
|
|
IHC-P |
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use in 1/500 - 1/1000 |
|
ICC/IF |
1/100.
For unpurified use in 1/500 - 1/1000 |
|
Indirect ELISA |
Use a concentration of 0.002 - 2 µg/ml.
|
特記事項 |
---|
Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use in 1/100 - 1/1000 |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified use in 1/500 - 1/1000 |
ICC/IF
1/100. For unpurified use in 1/500 - 1/1000 |
Indirect ELISA
Use a concentration of 0.002 - 2 µg/ml. |
ターゲット情報
-
機能
Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells. -
組織特異性
Expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. Also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma. -
配列類似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3590 Human
- Entrez Gene: 16157 Mouse
- Omim: 600939 Human
- SwissProt: Q14626 Human
- SwissProt: Q64385 Mouse
- Unigene: 591088 Human
-
別名
- CRSDA antibody
- hCG_2011440 antibody
- I11RA_HUMAN antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling IL-11RA with purified ab125015 at 1:250 dilution (3.2 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) ab97051
was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling IL-11RA with Purified ab125015 at 1:100 dilution (8.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
All lanes : Anti-IL-11RA antibody [EPR5446] (ab125015) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lane 3 : M1 (mouse myeloid leukemia myeloblast) whole cell lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Mouse testis tissue lysate
Lane 6 : Mouse thymus tissue lysate
Lane 7 : Mouse lymph node tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 42,45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression pattern is consistent with what has been described in the literature (PMID: 26876177).
Blocking Buffer and concentration: 5% NFDM/TBST
-
All lanes : Anti-IL-11RA antibody [EPR5446] (ab125015) at 1/10000 dilution (Purified)
Lane 1 : A673 (Human muscle Ewing's Sarcoma ) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysates
Lane 4 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling IL-11RA with Purified ab125015 at 1/80 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
ELISA analysis of Human IL-11RA recombinant protein at 1000 ng/mL with ab125015. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
-
Overlay histogram showing K562 cells stained with unpurified ab125015 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125015, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
All lanes : Anti-IL-11RA antibody [EPR5446] (ab125015) at 1/1000 dilution (Unpurified)
Lane 1 : 293T lysate
Lane 2 : Saos2 lysate
Lane 3 : A673 lysate
Lane 4 : PC3 lysate
Lane 5 : Hut78 lysate
Lane 6 : K562 lysate
Lane 7 : TF1 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Unpurified ab125015 at 1/500 staining IL-11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab125015 は 3 報の論文で使用されています。
- Ng B et al. IL11 Activates Pancreatic Stellate Cells and Causes Pancreatic Inflammation, Fibrosis and Atrophy in a Mouse Model of Pancreatitis. Int J Mol Sci 23:N/A (2022). PubMed: 35408908
- Dong J et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
- Wang D et al. Hepatectomy promotes recurrence of liver cancer by enhancing IL-11-STAT3 signaling. EBioMedicine 46:119-132 (2019). PubMed: 31375423