製品の概要

  • 製品名Anti-IL10RA antibody
    IL10RA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IL10RA
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human IL10RA.

    (Peptide available as ab109885.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HUVEC whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Receptor for IL10; binds IL10 with a high affinity.
  • 組織特異性Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.
  • 関連疾患Inflammatory bowel disease 28
  • 配列類似性Belongs to the type II cytokine receptor family.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD210 antibody
    • CDw210a antibody
    • CDw210a antigen antibody
    • HIL 10R antibody
    • HIL10R antibody
    • I10R1_HUMAN antibody
    • IL 10R 1 antibody
    • IL 10R A antibody
    • IL 10R antibody
    • IL 10R1 antibody
    • IL-10 receptor subunit alpha antibody
    • IL-10R subunit 1 antibody
    • IL-10R subunit alpha antibody
    • IL-10R1 antibody
    • IL-10RA antibody
    • IL10R antibody
    • IL10R1 antibody
    • IL10RA antibody
    • Interleukin 10 receptor alpha antibody
    • Interleukin 10 receptor alpha chain antibody
    • Interleukin-10 receptor subunit 1 antibody
    • Interleukin-10 receptor subunit alpha antibody
    see all

Anti-IL10RA antibody 画像

  • Anti-IL10RA antibody (ab94811) at 1 µg/ml + HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab94811 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94811 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IL10RA antibody (ab94811) 使用論文

ab94811 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"