The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Receptor for IL10; binds IL10 with a high affinity.
Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.