製品の概要

  • 製品名Anti-IL10 antibody [JES5-2A5]
    IL10 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [JES5-2A5] to IL10
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Fr, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant full length protein (Mouse)

  • 特記事項

    ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーpH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.14% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • 精製度Affinity purified
  • 一次抗体 備考ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.
  • ポリ/モノモノクローナル
  • クローン名JES5-2A5
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa.

We recommend incubating the membrane overnight in primary antibody.

Flow Cyt Use at an assay dependent concentration.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性Belongs to the IL-10 family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody [JES5-2A5] 画像

  • All lanes : Anti-IL10 antibody [JES5-2A5] (ab33471) at 1/500 dilution

    Lane 1 : Mouse liver whole cell lysate (treated with LPS 5mg/kg)
    Lane 2 : Mouse hepatocytes (Treated with LPS 100 ng/ml)

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-rat (HRP) at 1/1000 dilution
    Developed using the ECL technique

    Observed band size : 17 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa (possible non-specific binding).

    Exposure time : 45 seconds

    This image is courtesy of an anonymous abreview.

    Blocked with 5% milk for 18 hours at 4°C.

    See Abreview

  • Immunohistochemistry (Frozen sections) analysis of rat  brain tissue sections labeling IL10 with ab33471 at 1/1000 dilution. The tissue was fixed with paraformaldehyde and permeabilized with 0.1 M PBS with 3% Triton X. The tissue was blocked with 10% serum for 1 hour at 24°C, followed by incubation with Anti-IL10 antibody [JES5-2A5] (ab33471) in 0.1M PBST with 10% donkey serum for 24 hours at 4°C. Alexa Fluor 488® secondary antibody was used at 1/1000 dilution. The IL-10 was visible in the M2 macrophages in the choroid plexus.  Nucleus was stained with DAPI.

Anti-IL10 antibody [JES5-2A5] (ab33471) 使用論文

This product has been referenced in:
  • Yao L  et al. Notch-1 Signaling Regulates Microglia Activation via NF-?B Pathway after Hypoxic Exposure In Vivo and In Vitro. PLoS One 8:e78439 (2013). WB ; Mouse . Read more (PubMed: 24223152) »
  • Fyhrquist N  et al. Foxp3+ cells control Th2 responses in a murine model of atopic dermatitis. J Invest Dermatol 132:1672-80 (2012). Mouse . Read more (PubMed: 22402436) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse liver, mouse hepatocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Treatment LPS 5mg/kg iv for liver, 100 ng/ml for hepatocytes
Specification mouse liver, mouse hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 23 2017

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Jun 25 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 13 2014

Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Thank you for contacting us.

We haven't tested this antibody in paraffin embedded tissue sections (IHC-P), only frozen sections from mouse. However, IL10 is produced and secreted by a variety of cell lines, including T-cells, macrophages, ma...

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We typically load 10 - 20 nanograms of purified recombinant protein in gels for western blots. I have contacted the laboratory to find out the exact amount that was used for the blot on the datasheet but I expect it will be within that range.

Thank you for contacting us.

We recommend ab18412.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply and for sending your protocol information and image. We do guarantee ab33471 to work in Western blot with mouse lysates, so we are happy to issue you a refund for this purchase. I have requested the refund to our accoun...

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According to our records, ab33471 was proving difficult to use in IHC and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order for us to resolve the problem. If the ...

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Thank you for contacting us, and I am sorry that this antibody has not produced expected results. We are happy to offer a refund if a product does not work in a tested species and application within 6 months of purchase. Do you have your order number? ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"